Difference between revisions of "Part:BBa K3081040"
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__NOTOC__
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<partinfo>BBa_K3081059 short</partinfo>
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dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.
 
dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3081040 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3081040 parameters</partinfo>
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Revision as of 17:55, 19 October 2019


dCas9 J23119 M+ with the thermo-sensitive transcription factor TCI42

dCas9 based replication control on p15A origin plasmid copy number——top strand of initiation site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961