Difference between revisions of "Part:BBa K112401:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | ==pBca1256-K112401== | ||
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+ | <pre> | ||
+ | Biobricking of promoter for recA protein | ||
+ | PCR recA-F and recA-R on MG1655 (317 bp, EcoR1/BamH1) | ||
+ | Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) | ||
+ | Product is pBca1256-K112401 | ||
+ | ---------------------------- | ||
+ | cb0003-recA-F Forward Biobricking cgataGAATTCatgAGATCTagagaagcctgtcggcaccg | ||
+ | cb0004-recA-R Reverse Biobricking cgtagGGATCCcccagtgctgccgccaacgc | ||
+ | </pre> | ||
Latest revision as of 04:08, 24 October 2008
Promoter for recA gene - SOS and Ultrasound Sensitive
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pBca1256-K112401
Biobricking of promoter for recA protein PCR recA-F and recA-R on MG1655 (317 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112401 ---------------------------- cb0003-recA-F Forward Biobricking cgataGAATTCatgAGATCTagagaagcctgtcggcaccg cb0004-recA-R Reverse Biobricking cgtagGGATCCcccagtgctgccgccaacgc
Source
Cloned from E. coli MG1655 genome.