Difference between revisions of "Part:BBa K3007000"
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It is a special DNA sequence which can interact with HucR protein and repress the expression of downstream gene when uric acid is absent. If uric acid presents, the formation of HucR-hucO complex can be reduced and restore downstream gene expression. In our design, we used the HucR-hucO system as a detector for uric acid. | It is a special DNA sequence which can interact with HucR protein and repress the expression of downstream gene when uric acid is absent. If uric acid presents, the formation of HucR-hucO complex can be reduced and restore downstream gene expression. In our design, we used the HucR-hucO system as a detector for uric acid. | ||
| − | + | ===Contribution=== | |
| − | === | + | <div> |
| − | + | Group: QHFZ-China iGEM 2019 | |
| + | <br> | ||
| + | Author: Cheng Li | ||
| + | <br> | ||
| + | Design: | ||
| + | <br> | ||
[[File:T--QHFZ-China--BBa_K3007000_1.png|200px|thumb|left|Figure 1. Schematic cartoon of the DNA construct of BBa_K3007000]] | [[File:T--QHFZ-China--BBa_K3007000_1.png|200px|thumb|left|Figure 1. Schematic cartoon of the DNA construct of BBa_K3007000]] | ||
| + | <br> | ||
| + | Documentation: | ||
| + | <br> | ||
| + | |||
| + | |||
| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
Revision as of 16:47, 19 October 2019
HucR binding site
It is a special DNA sequence which can interact with HucR protein and repress the expression of downstream gene when uric acid is absent. If uric acid presents, the formation of HucR-hucO complex can be reduced and restore downstream gene expression. In our design, we used the HucR-hucO system as a detector for uric acid.
Contribution
Group: QHFZ-China iGEM 2019
Author: Cheng Li
Design:
Documentation:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]