Difference between revisions of "Part:BBa K3081003"
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A fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. | A fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. | ||
A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. | A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. | ||
| − | The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose.This part is an improvement of dCas9-linker-sfGFP(<partinfo>BBa_K3081002</partinfo>), in which we add a degradation signal peptide ssrA to sfGFP. | + | The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose.This part is an improvement of pBAD dCas9-linker-sfGFP(<partinfo>BBa_K3081002</partinfo>), in which we add a degradation signal peptide ssrA to sfGFP. |
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Revision as of 16:32, 19 October 2019
pBAD-dCas9-linker-sfGFP-ssrA
A fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose.This part is an improvement of pBAD dCas9-linker-sfGFP(BBa_K3081002), in which we add a degradation signal peptide ssrA to sfGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 2321 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 4600 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 5369