Difference between revisions of "Part:BBa K2213012:Experience"
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===User Reviews===
 
===User Reviews===
 
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<I>Alexander_Schanne</I>
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<I>Alexander_Schannee</I>
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We have changed the original RBS to the very weak RBS J61100 in order to have a lower base expression level. Additionally we replaced the LacI promoter with the araBad promoter since the LacI is known to be leaky and we wanted to keep the expression of all proteins under more tight control.
 
We have changed the original RBS to the very weak RBS J61100 in order to have a lower base expression level. Additionally we replaced the LacI promoter with the araBad promoter since the LacI is known to be leaky and we wanted to keep the expression of all proteins under more tight control.
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Revision as of 18:31, 19 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2213012

User Reviews

UNIQ9274c06cd9b836f0-partinfo-00000000-QINU

Alexander_Schanne


We have changed the original RBS to the very weak RBS J61100 in order to have a lower base expression level. Additionally we replaced the LacI promoter with the araBad promoter since the LacI is known to be leaky and we wanted to keep the expression of all proteins under more tight control.

UNIQ9274c06cd9b836f0-partinfo-00000001-QINU