Difference between revisions of "Part:BBa K3081063"
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"This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IHF binding region of plasmid replication origin pSC101,using in the CRISPRri system<partinfo>BBa_K3081007</partinfo> to control the  plasmid copy number."
 
"This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IHF binding region of plasmid replication origin pSC101,using in the CRISPRri system<partinfo>BBa_K3081007</partinfo> to control the  plasmid copy number."
  
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<center>
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https://2019.igem.org/wiki/images/c/cf/T--Peking--plasmid_copy_number_pL01.png
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</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:18, 21 October 2019


pBAD-dCas9-J23119-pL05

"This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IHF binding region of plasmid replication origin pSC101,using in the CRISPRri systemBBa_K3081007 to control the plasmid copy number."

T--Peking--plasmid_copy_number_pL01.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961