Difference between revisions of "Part:BBa K3196018"
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<partinfo>BBa_K3196018 short</partinfo>
 
<partinfo>BBa_K3196018 short</partinfo>
  
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PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. pelA can catalyze pectin.
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PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. pelA can synthetic pectin lyase.
  
 
<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
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This is a four section for degrade and transfer lignin part.
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This is a four section for degrade and transfer pectin part.
 
[[File:T--HUST--China--2019-PHO1pelA.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1pelA]]
 
[[File:T--HUST--China--2019-PHO1pelA.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1pelA]]
  
 
<h1>'''DNA Gel Electrophoretic'''</h1>
 
<h1>'''DNA Gel Electrophoretic'''</h1>
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After we link PHO1 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
+
After we link PHO1 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1100 bp which means the PCR is successful.
 
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
  

Revision as of 06:45, 20 October 2019


AOX1-Kozak-PHO1-pelA-His tag-AOX1 Terminator

PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. pelA can synthetic pectin lyase.

Characterization

This is a four section for degrade and transfer pectin part.

Figure1. T--HUST--China--2019-PHO1pelA

DNA Gel Electrophoretic

After we link PHO1 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1100 bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1767
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]