Difference between revisions of "Part:BBa K3196016"
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<partinfo>BBa_K3196016 short</partinfo> | <partinfo>BBa_K3196016 short</partinfo> | ||
| − | The | + | The FLO10 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. pelA can catalyze pectin. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
| − | This is a four section for degrade and transfer | + | This is a four section for degrade and transfer pectin part. |
[[File:T--HUST--China--2019-FLO10pelA.jpg |400px|thumb|center|Figure1. T--HUST--China--2019-FLO10pelA]] | [[File:T--HUST--China--2019-FLO10pelA.jpg |400px|thumb|center|Figure1. T--HUST--China--2019-FLO10pelA]] | ||
Revision as of 05:46, 20 October 2019
AOX1-Kozak-FLO10-pelA-His tag-AOX1 Terminator
The FLO10 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. pelA can catalyze pectin.
Characterization
This is a four section for degrade and transfer pectin part.
DNA Gel Electrophoretic
After we link SUC2 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1787
Illegal BamHI site found at 937
Illegal BamHI site found at 948 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]