Difference between revisions of "Part:BBa K3196016"

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<partinfo>BBa_K3196016 short</partinfo>
 
<partinfo>BBa_K3196016 short</partinfo>
  
The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. pelA can catalyze pectin.
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The FLO10 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. pelA can catalyze pectin.
  
 
<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
This is a four section for degrade and transfer lignin part.
+
This is a four section for degrade and transfer pectin part.
 
[[File:T--HUST--China--2019-FLO10pelA.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-FLO10pelA]]
 
[[File:T--HUST--China--2019-FLO10pelA.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-FLO10pelA]]
  

Revision as of 05:46, 20 October 2019


AOX1-Kozak-FLO10-pelA-His tag-AOX1 Terminator

The FLO10 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. pelA can catalyze pectin.

Characterization

This is a four section for degrade and transfer pectin part.

Figure1. T--HUST--China--2019-FLO10pelA

DNA Gel Electrophoretic

After we link SUC2 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1787
    Illegal BamHI site found at 937
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]