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	<h1>'''Characterization'''</h1>		<h1>'''Characterization'''</h1>
−	This is a four section for degrade and transfer lignin part.	+	This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.[4] It can help the peptide chain of VP be transferred out cells. [[File:T--HUST--China--2019-FLO10-αproVP.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αproVP]]
−	[[File:T--HUST--China--2019-FLO10-αproVP.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αproVP]]	+
	<h1>'''DNA Gel Electrophoretic'''</h1>		<h1>'''DNA Gel Electrophoretic'''</h1>

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Revision as of 22:42, 19 October 2019

AOX1-Kozak-FLO10 pro-VP-His tag-AOX1 Terminator

FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze pectin.

Characterization

This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.[4] It can help the peptide chain of VP be transferred out cells.

DNA Gel Electrophoretic

After we link FLO10 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.

Sequence and Features