Revision as of 07:01, 20 October 2019

AOX1-Kozak-PHO1 pro-pelA-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze pectin.

Characterization

This is a four section for degrade and transfer pectin part.

Figure1. T--HUST--China--2019-PHO1-αpropelA

DNA Gel Electrophoretic

After we link PHO1 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1100 bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1968
Illegal BamHI site found at 937
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <h1>'''Characterization'''</h1>
-This is a four section for degrade and transfer lignin part.
+This is a four section for degrade and transfer pectin part.
 [[File:T--HUST--China--2019-PHO1-αpropelA.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1-αpropelA]]
 <h1>'''DNA Gel Electrophoretic'''</h1>
-After we link PHO1 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
+After we link PHO1 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1100 bp which means the PCR is successful.
 [[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]

Difference between revisions of "Part:BBa K3196019"

Revision as of 07:01, 20 October 2019

Characterization

DNA Gel Electrophoretic