Difference between revisions of "Part:BBa K3081113"

Revision as of 11:35, 21 October 2019

a 20bp sequence targeting the DNA unwinding element on E.coli oriC

The 20 bp sgRNA targets to the duplex unwinding element (DUE) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. For more detailed information, see BBa_K3081058

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]

Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
+<center>
+https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
+</center>
+<center>
+Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
+</center>
+Reference:
+[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
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