Difference between revisions of "Part:BBa K135001"
m |
|||
Line 2: | Line 2: | ||
<partinfo>BBa_K135001 short</partinfo> | <partinfo>BBa_K135001 short</partinfo> | ||
− | This is a fusion of the CpxR responsive promoter pCpxR (BBa_K135000), upstream of BBa_E0840. | + | This is a fusion of the CpxR responsive promoter pCpxR (BBa_K135000), upstream of BBa_E0840. It is not shown below in simple composite form as there was an extra bp in the linker sequence between BBa_K135000 and BBa_E0840 that the composite tool put in, which was not there in sequencing. |
+ | |||
+ | This part can be used as a visual assay for adhesion to hydrophobic surfaces in E.Coli. See part BBa_K135000 for an explanation of how this works and a paper reference. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 15:38, 20 October 2008
pCpxR-BBa_E0840 fusion
This is a fusion of the CpxR responsive promoter pCpxR (BBa_K135000), upstream of BBa_E0840. It is not shown below in simple composite form as there was an extra bp in the linker sequence between BBa_K135000 and BBa_E0840 that the composite tool put in, which was not there in sequencing.
This part can be used as a visual assay for adhesion to hydrophobic surfaces in E.Coli. See part BBa_K135000 for an explanation of how this works and a paper reference.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 727