Difference between revisions of "Part:BBa K135000:Experience"

Revision as of 15:50, 20 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K135000

We have used this part, fused upstream of BBa_E0840 (BBa_K135001), and have shown GFP activation in reponse to adhesion on glass slides. The conclusion that this is activated in response to adhesion follows from Ottos and Silhavys work (see main page description for reference), in which they demonstrated activation of a pCpxR-LacZ fusion in response to adhesion to glass microspheres. Filamentous bacteria fluoresced significantly, while it was hard to ascertain whether smaller bacteria were fluorescing. Due to time constraints, a better characterisation of the efficiency of the promoter could not be performed.

User Reviews

UNIQc79a6f2586f31c40-partinfo-00000000-QINU UNIQc79a6f2586f31c40-partinfo-00000001-QINU

@@ Line 4: / Line 4: @@
 ===Applications of BBa_K135000===
-We have used this part, fused upstream of the GFP from BBa_E0840, and have shown GFP activation in repsonse to adhesion on glass slides. The conclusion that this is activated in response to adhesion follows from Ottos and Silhavys work (see main page description for reference), in which they demonstrated activation in response to adhesion to glass microspheres. Filamentous bacteria fluoresced significantly, while it was hard to ascertain whether smaller bacteria were fluorescing. Due to time constraints, a better characterisation of the efficiency of the promoter could not be performed.
+We have used this part, fused upstream of BBa_E0840 (BBa_K135001), and have shown GFP activation in reponse to adhesion on glass slides. The conclusion that this is activated in response to adhesion follows from Ottos and Silhavys work (see main page description for reference), in which they demonstrated activation of a pCpxR-LacZ fusion in response to adhesion to glass microspheres. Filamentous bacteria fluoresced significantly, while it was hard to ascertain whether smaller bacteria were fluorescing. Due to time constraints, a better characterisation of the efficiency of the promoter could not be performed.
 ===User Reviews===