Difference between revisions of "Part:BBa K3081110"

Line 5: Line 5:
 
The 19 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box using a 19bp sgRNA, alleviation of severe arrest and inhibition to the genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
 
The 19 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box using a 19bp sgRNA, alleviation of severe arrest and inhibition to the genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
  
 +
 +
<center>
 +
https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
 +
</center>
 +
<center>
 +
Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
 +
</center>
 +
 +
 +
Reference:
 +
[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:29, 21 October 2019


a 19bp sequence targeting the R1(top strand) DnaA box on E.coli oriC

The 19 bp sgRNA targets to the R1+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1+ is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1+ box using a 19bp sgRNA, alleviation of severe arrest and inhibition to the genome replication initiation is achieved. For more detailed information, see BBa_K3081058


T--Peking--box.png

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]


Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]