Difference between revisions of "Part:BBa K2983017"

Revision as of 13:43, 19 October 2019 (view source) Anthor91 (Talk | contribs) (→‎Usage and Biology==) ← Older edit		Revision as of 14:49, 19 October 2019 (view source) Anthor91 (Talk | contribs) (→‎Usage and Biology) Newer edit →
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	This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.		This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
−	=Usage and Biology=	+	===Usage and Biology===
		+	[[File:T--Evry Paris-Saclay--Loop-Sites.png|300px|thumb| Figure 1. BsaI and SapI restriction sites (adapted from [1])]] The Loop Type IIS cloning sites (triangles in figures 1 and 2) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figures 1 and 2).
−	The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the Loop F-Omega is as fellow : GGTCTCACGCTAGGTTGAAGAGC	+	[[File:T--Evry Paris-Saclay--Loop F-Omega.png|300px|thumb|left| Figure2. Loop F-Omega structure]]
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−	[[File:T--Evry Paris-Saclay--Loop F-Omega.png|300px|thumb|left| Figure1: Loop F-Omega structure]]	+
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	Yellow circle: overhang formed by BasI  after cutting (CGCT)		Yellow circle: overhang formed by BasI  after cutting (CGCT)
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		+	This part was used to define the Loop assembly system dedicated to the oleaginous yeast ''Yarrowia lipolytica'' (for details visit https://2019.igem.org/Team:Evry_Paris-Saclay/Design)
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		+	===References===
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		+	[1] Pollak B, Cerda A, Delmans M, Álamos S, Moyano T, West A, Gutiérrez RA, Patron NJ, Federici F, Haseloff J. Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol (2019) 222, 628-640.

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Revision as of 14:49, 19 October 2019

Loop F-Omega

This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.

Usage and Biology

The Loop Type IIS cloning sites (triangles in figures 1 and 2) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figures 1 and 2).

Blue triangle: SapI fixation site (GCTCTTC)

Red triangle : BsaI fixation site (GAGACC)

Blue circle : overhang formed by SapI after cutting (GGT)

Yellow circle: overhang formed by BasI after cutting (CGCT)

This part was used to define the Loop assembly system dedicated to the oleaginous yeast Yarrowia lipolytica (for details visit https://2019.igem.org/Team:Evry_Paris-Saclay/Design)

References

[1] Pollak B, Cerda A, Delmans M, Álamos S, Moyano T, West A, Gutiérrez RA, Patron NJ, Federici F, Haseloff J. Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol (2019) 222, 628-640.

Sequence and Features