Difference between revisions of "Part:BBa K3196009"

 
Line 5: Line 5:
 
signal peptide suitable for expression in  pichia pastoris
 
signal peptide suitable for expression in  pichia pastoris
 
<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
The signal peptide PHO5 was combined with the guiding peptide sequence of saccharomyces cerevisiae to form the signal peptide PHO5- αpro.
+
The signal peptide PHO5 was combined with the guiding peptide sequence of saccharomyces cerevisiae to form the signal peptide PHO5- αpro.PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times.
  
  

Latest revision as of 05:49, 20 October 2019


PHO1 pro

signal peptide suitable for expression in pichia pastoris

Characterization

The signal peptide PHO5 was combined with the guiding peptide sequence of saccharomyces cerevisiae to form the signal peptide PHO5- αpro.PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]