Difference between revisions of "Part:BBa K2983012"
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− | Blue triangle: SapI fixation site () | + | Blue triangle: SapI fixation site (GCTCTTC) |
− | Red triangle : BsaI fixation site () | + | Red triangle : BsaI fixation site (GAGACC) |
+ | |||
+ | Blue circle : overhang formed by SapI after cutting (GCA) | ||
+ | |||
+ | red circle: overhang formed by BasI after cutting (GGAG) | ||
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Revision as of 13:26, 19 October 2019
Loop Beta-A
This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
Usage and Biology
The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the Loop Beta-A is as fellow : GCTCTTCAGCAAGGAGTGAGACC
Blue triangle: SapI fixation site (GCTCTTC)
Red triangle : BsaI fixation site (GAGACC)
Blue circle : overhang formed by SapI after cutting (GCA)
red circle: overhang formed by BasI after cutting (GGAG)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 18
Illegal SapI site found at 1