Difference between revisions of "Part:BBa K2963030"

Latest revision as of 10:20, 20 October 2019

Tac Promoter (including two operator genes(lacO))

Tac promoter is a hybrid of two operons: the trp and lac operons. Tac promoter is inducible by IPTG and commonly used in Corynebacterium glutamicum for overproduction of proteins. Corynebacterium glutamicum constitutively produces the LacI protein, a strong Ptac promoter repressor which can bind with lac operator. In our experiment, we add another operator gene to tac promoter in order to regulate the expression of the racE gene to produce γ-PGA with different D/L-glutamate monomer ratios.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2963030 short</partinfo>
+Tac promoter is a hybrid of two operons: the trp and lac operons. Tac promoter is inducible by IPTG and commonly used in <i>Corynebacterium glutamicum</i> for overproduction of proteins. <i>Corynebacterium glutamicum</i> constitutively produces the LacI protein, a strong Ptac promoter repressor which can bind with lac operator. In our experiment, we add another operator gene to tac promoter in order to regulate the expression of the <i>racE</i> gene to produce γ-PGA with different D/L-glutamate monomer ratios.
-This promoter is a hybrid of two operons: the trp and lac operons. This promoter is inducible by IPTG and commonly used in <i>Corynebacterium glutamicum</i> for overproduction of proteins. <i>Corynebacterium glutamicum</i> constitutively produces the LacI protein, a strong Ptac promoter repressor which can bind with lac operator. In our experiment, we add two lac operators in order to regulate the expression of the <i>racE</i> gene to produce γ-PGA with different D/L-glutamate monomer ratios.
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