Difference between revisions of "Part:BBa K3278000"
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This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation.
 
This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation.
  
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<b><font size="3"> 0 hour:</font></b>
 
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pDawn with YF2: BBa_K3278006 (good)</p>
 
pDawn with YF2: BBa_K3278006 (good)</p>
  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:38, 20 October 2019


pDawn-mEGFP

This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation.


0 hour: <img src="447px-T--ShanghaiTech_China--characterization_13%28pme_0h%29.png" alt="pme_0h" width="395" height="635">

6 hour: <img src="800px-T--ShanghaiTech_China--characterization_14%28pme_6h%29.png" alt="pme_6h" width="790" height="635">

12 hour: <img src="800px-T--ShanghaiTech_China--characterization_15%28pme_12h%29.png" alt="pme_12h" width="790" height="635">

24 hour: <img src="800px-T--ShanghaiTech_China--characterization_16%28pme_24h%29.png" alt="pme_24h" width="790" height="635">

30 hour: <img src="800px-T--ShanghaiTech_China--characterization_17%28pme_30h%29.png" alt="pme_30h" width="790" height="635">

36 hour: <img src="800px-T--ShanghaiTech_China--characterization_18%28pme_36h%29.png" alt="pme_36h" width="790" height="635">


To see more changes on pDawn, go to the websites below.

pDawn+mEGFP: BBa_K3278000

pDawn with T7 promoter: BBa_3278001

pDawn with mtac promoter: BBa_K3278002 (good)

pdawn with tac promoter: BBa_K3278005

pDawn with YF2: BBa_K3278006 (good)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
    Illegal NgoMIV site found at 178
    Illegal NgoMIV site found at 272
    Illegal NgoMIV site found at 565
    Illegal NgoMIV site found at 1059
    Illegal NgoMIV site found at 1077
    Illegal NgoMIV site found at 1167
    Illegal AgeI site found at 397
    Illegal AgeI site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1620
    Illegal BsaI.rc site found at 508