Difference between revisions of "Part:BBa K3196024"
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<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
 
This is a four section for degrade and transfer lignin part.
 
This is a four section for degrade and transfer lignin part.
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[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
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[[File:T--HUST--China--2019-PHO1-αproVP.jpg‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1-αproVP]]
  
 
<h1>'''DNA Gel Electrophoretic'''</h1>
 
<h1>'''DNA Gel Electrophoretic'''</h1>
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[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
  
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<h1>'''SDS-PAGE'''</h1>
 
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We run the SDS-PAGE to check whether PHO1 αpro help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.
 
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[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 
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<h1>'''Enzyme Activity'''</h1>
 
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We use manganese and hydrogen peroxide to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.
 
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[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 
  
 
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Revision as of 15:37, 19 October 2019


AOX1-Kozak-PHO1 pro-VP-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze pectin.

Characterization

This is a four section for degrade and transfer lignin part.

Figure1. T--HUST--China--2019-PHO1-αproVP

DNA Gel Electrophoretic

After we link PHO1 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]