Difference between revisions of "Part:BBa K3081006"
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, in which polyA sequence is the control sgRNA targeted no binding site of E.coli genome replication initiation region, OriC.
 
This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, in which polyA sequence is the control sgRNA targeted no binding site of E.coli genome replication initiation region, OriC.
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For more detailed information, see <a href="https://parts.igem.org/Part:BBa_K3081058">BBa_K3081058</a>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:02, 19 October 2019


pBAD-dCas9-J23119-polyA

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, in which polyA sequence is the control sgRNA targeted no binding site of E.coli genome replication initiation region, OriC.

For more detailed information, see <a href="https://parts.igem.org/Part:BBa_K3081058">BBa_K3081058</a>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961