Difference between revisions of "Part:BBa K3105668"
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Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for <I>Pichia pastoris</I>. | Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for <I>Pichia pastoris</I>. | ||
| − | HRP is | + | HRP is commonly used as a antibody-conjugate and is detected by chemiluminescence. |
The underlying reaction mechanism is described below: <br> | The underlying reaction mechanism is described below: <br> | ||
| − | HRP+ H2O2 = | + | HRP+ H2O2 = oxidized substrate |
<br> | <br> | ||
Revision as of 20:57, 19 October 2019
Horseradish peroxidase
Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for Pichia pastoris.
HRP is commonly used as a antibody-conjugate and is detected by chemiluminescence.
The underlying reaction mechanism is described below:
HRP+ H2O2 = oxidized substrate
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 880
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 961
- 1000COMPATIBLE WITH RFC[1000]