Difference between revisions of "Part:BBa K3317031"
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<partinfo>BBa_K3317031 short</partinfo> | <partinfo>BBa_K3317031 short</partinfo> | ||
| − | Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) S. enterica LT2 (NC_003197.1) ribosome re-initiation site 1 and ribosome re-initiation site 2 was placed between pduJK and pduN was placed between pduAB and pduJK,this sequence is contained in plasmid pMCY30 generously provided by Dr. Mimi C. Yung | + | Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) S. enterica LT2 (NC_003197.1) ribosome re-initiation site 1 and ribosome re-initiation site 2 was placed between pduJK and pduN was placed between pduAB and pduJK,this sequence is contained in plasmid pMCY30 generously provided by <a href=https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0685-x#Sec23">Dr. Mimi C. Yung</a> |
The plasmid contains the proteins pduA (<partinfo>K3317008</partinfo>) pduB(<partinfo>K3317009</partinfo>) pduJ | The plasmid contains the proteins pduA (<partinfo>K3317008</partinfo>) pduB(<partinfo>K3317009</partinfo>) pduJ | ||
(<partinfo>K3317010</partinfo>) pduK (<partinfo>K3317011</partinfo>) and pduN (<partinfo>K3317012</partinfo>) | (<partinfo>K3317010</partinfo>) pduK (<partinfo>K3317011</partinfo>) and pduN (<partinfo>K3317012</partinfo>) | ||
Revision as of 23:22, 18 October 2019
BMC shell proteins ABJKN from S. enterica LT2
Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) S. enterica LT2 (NC_003197.1) ribosome re-initiation site 1 and ribosome re-initiation site 2 was placed between pduJK and pduN was placed between pduAB and pduJK,this sequence is contained in plasmid pMCY30 generously provided by <a href=https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0685-x#Sec23">Dr. Mimi C. Yung</a> The plasmid contains the proteins pduA (BBa_K3317008) pduB(BBa_K3317009) pduJ (BBa_K3317010) pduK (BBa_K3317011) and pduN (BBa_K3317012)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 41
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1761
Illegal BamHI site found at 1900 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 41
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 41
Illegal NgoMIV site found at 1388
Illegal AgeI site found at 1655
Illegal AgeI site found at 1691 - 1000COMPATIBLE WITH RFC[1000]