Difference between revisions of "Part:BBa K137079"
 
Line 14: Line 14:
 
|DH10B||Empty||<div align="right">0.8</div>||<div align="right">1.6</div>
 
|DH10B||Empty||<div align="right">0.8</div>||<div align="right">1.6</div>
 
|-
 
|-
−
|JI377||Empty||<div align="right">0.0</div>||<div align="right">0.2</div>
+
|SN0037||Empty||<div align="right">0.0</div>||<div align="right">0.2</div>
 
|-
 
|-
−
|JI377||K137079, -AHL||<div align="right">0.3</div>||<div align="right">1.0</div>
+
|SN0037||K137079, -AHL||<div align="right">0.3</div>||<div align="right">1.0</div>
 
|-
 
|-
−
|JI377||K137079, +AHL||<div align="right">3.0</div>||<div align="right">10.0</div>
+
|SN0037||K137079, +AHL||<div align="right">3.0</div>||<div align="right">10.0</div>
 
|}
 
|}
  

Latest revision as of 17:47, 17 October 2008

AHL inducible expression of KatG

This part produces an E. coli catalase (katG) tagged with an LAA degradation tag upon induction with an acylhomoserine lactone.


Usage and Biology

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/MIC_Assay minimum inhibitory concentration (MIC) assay], K137079 in pSB1A2 in SN0037 (ΔkatGΔkatE) was able to increase the MIC of SN0037 as shown below:

Strain Plasmid Highest H2O2 concentration survived (mM) Lowest H2O2 concentration inhibited (mM)
DH10B Empty
0.8
1.6
SN0037 Empty
0.0
0.2
SN0037 K137079, -AHL
0.3
1.0
SN0037 K137079, +AHL
3.0
10.0

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1316
    Illegal BglII site found at 2251
    Illegal BglII site found at 2378
    Illegal BamHI site found at 2168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005