Difference between revisions of "Part:BBa K137018"
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===Usage and Biology===
 
===Usage and Biology===
−
When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], the response curve is shown below. 100nM AHL caused very slow growth, leading to poor protein expression.
+
When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], K137018 in pSB1A2 in DH10B produces the response curve shown below. 100nM AHL caused very slow growth, leading to poor protein expression.
  
 
[[Image:K137018.gif]]
 
[[Image:K137018.gif]]

Revision as of 17:21, 17 October 2008

AHL receiver + GFP

This parts produces GFP in response to detecting an acylhomoserinelactone (AHL). It was made to check that the RBS behind the luxR reciever was functional.


Usage and Biology

When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], K137018 in pSB1A2 in DH10B produces the response curve shown below. 100nM AHL caused very slow growth, leading to poor protein expression.

K137018.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005
    Illegal BsaI.rc site found at 1732