Difference between revisions of "Part:BBa K3110046"

 
Line 5: Line 5:
 
\FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th  position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to  RPMrel. We amplified the FimHRPMrel from this existing part, and this can be used  in combination with different tags and/or promoter/RBS sequences.  
 
\FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th  position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to  RPMrel. We amplified the FimHRPMrel from this existing part, and this can be used  in combination with different tags and/or promoter/RBS sequences.  
  
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->

Latest revision as of 12:27, 21 October 2019


FimH 136KO-RPMrel

\FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to RPMrel. We amplified the FimHRPMrel from this existing part, and this can be used in combination with different tags and/or promoter/RBS sequences.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 496
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]