Difference between revisions of "Part:BBa K2984063"

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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The backbone is desgined for MoClo level 2 cloning. Together with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984003">L1a</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984005">L1b</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984002"> L1c </a> three different level 1 constrcuts can be inserted into the level 2 backbone. The backbone contains a transcriptional unit, which we call selction-cassette. This unit consists of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008"> Psad </a> promoter, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984006"> paromomycin resistance paroR </a> and  the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018"> Rbcs2 </a> terminator. It is flanked by a N-terminal BamHI and a C-terminal XhoI restriction site to allow the exchange of the selection.cassette. Herefore our <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984056"> hygromycin construct </a> or <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984037"> ptxD construct </a>
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The backbone is desgined for MoClo level 2 cloning. Together with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984003">L1a</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984005">L1b</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984002"> L1c </a> three different level 1 constructs can be inserted into the level 2 backbone. The backbone contains a transcriptional unit, which we call selection-cassette. This unit consists of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008"> Psad </a> promoter, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984006"> paromomycin resistance paroR </a> and  the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018"> Rbcs2 </a> terminator. It is flanked by an N-terminal <i>BamHI</i> and a C-terminal <i>XhoI</i> restriction site to allow the exchange of the selection-cassette. Herefore our <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984056"> hygromycin construct </a> or <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984037"> ptxD construct </a>
could be inserted instead. The selction-cassette benfits the transofrmation rate of Chlamydomonas since it makes co-transformations obsolete. It is not necessary to clone all three level 1 construct into the backbone. It could be redused to one or two using one of our  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984062"> Level 2 Linker </a> </html>
+
could be inserted instead. The selection-cassette benfits the transformation rate of Chlamydomonas since it makes co-transformations obsolete. It is not necessary to clone all three level 1 construct into the backbone. It could be redused to one or two using one of our  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984062"> Level 2 Linker </a> </html>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 23:11, 19 October 2019


Phytobrick Level 2 backbone (with C.reinhardtii paromomycin marker)

Level 2 backbone


Usage and Biology

This part is a part of the Chlamy-HUB-Collection. The backbone is desgined for MoClo level 2 cloning. Together with the L1a, L1b and L1c three different level 1 constructs can be inserted into the level 2 backbone. The backbone contains a transcriptional unit, which we call selection-cassette. This unit consists of the Psad promoter, the paromomycin resistance paroR and the Rbcs2 terminator. It is flanked by an N-terminal BamHI and a C-terminal XhoI restriction site to allow the exchange of the selection-cassette. Herefore our hygromycin construct or ptxD construct could be inserted instead. The selection-cassette benfits the transformation rate of Chlamydomonas since it makes co-transformations obsolete. It is not necessary to clone all three level 1 construct into the backbone. It could be redused to one or two using one of our Level 2 Linker

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 1121
    Illegal XhoI site found at 3012
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 189
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.