Difference between revisions of "Part:BBa K2984018"
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'''Measurement'''
 
'''Measurement'''
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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==References==
 
==References==
 
<ol>
 
<ol>
 
<li>
 
<li>
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Loera‐Quezada, M. M., Leyva‐González, M. A., Velázquez‐Juárez, G., Sanchez‐Calderón, L., Do Nascimento, M., López‐Arredondo, D., & Herrera‐Estrella, L. (2016). A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae. Plant biotechnology journal, 14(10), 2066-2076.
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Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432.  
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</li>
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<li>
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White, A. K., & Metcalf, W. W. (2007). Microbial metabolism of reduced phosphorus compounds. Annu. Rev. Microbiol., 61, 379-400.
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</li>
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<li>
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White, A. K., & Metcalf, W. W. (2007). Microbial metabolism of reduced phosphorus compounds. Annu. Rev. Microbiol., 61, 379-400.
+
−
</li>
+
−
<li>
+
−
Loera-Quezada, M. M., Leyva-González, M. A., López-Arredondo, D., & Herrera-Estrella, L. (2015). Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae. Plant Science, 231, 124-130.
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</li>
 
</li>
 
</ol>
 
</ol>

Revision as of 19:14, 18 October 2019


Terminator.png

RbcS2 - Terminator B5-C1

  • This part is a part of the Chlamy-HUB-Collection. To terminate the transcription of a gene a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a Chlamydomonas reinhardtii native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Secondary Structure

File:Mfold-K2984018-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

References

  1. Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432.