Difference between revisions of "Part:BBa K2982000"
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A coding sequence for wild type PETase from Ideonella sakainesis. It is codon optimized for Escherichia coli. Can serve as a standard for other experiment related to PETase.
 
A coding sequence for wild type PETase from Ideonella sakainesis. It is codon optimized for Escherichia coli. Can serve as a standard for other experiment related to PETase.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2982000 parameters</partinfo>
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<partinfo>BBa_K2982005 parameters</partinfo>
 
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Revision as of 12:23, 21 October 2019


Coding sequence for S245I IsPETase double mutant

A coding sequence for wild type PETase from Ideonella sakainesis. It is codon optimized for Escherichia coli. Can serve as a standard for other experiment related to PETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 348
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]