Difference between revisions of "Part:BBa K2984004"

Revision as of 16:46, 18 October 2019

Red fluorescent protein

This part is a part of the Chlamy-HUB-Collection. Red florurescent protein designed for visual screening of E.coli colonies. The red color can be seen with the naked eye, which makes this part especially useful for screening.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 171
Illegal AgeI site found at 283
1000
COMPATIBLE WITH RFC[1000]

Characterization

colonies_total — Fig.1 - This part was used for our level 1 c backbone. This image shows a successful transformation of the level 1 c backbone with RFP in *E. coli*, which makes the colonies red.

@@ Line 4: / Line 4: @@
 <html>
-This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Red florurescent protein designed for visual screening of E.coli colonies
+This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Red florurescent protein designed for visual screening of E.coli colonies. The red color can be seen with the naked eye, which makes this part especially useful for screening.
 </html>
@@ Line 20: / Line 20: @@
 <figure>
 <img src="https://static.igem.org/mediawiki/parts/3/3d/L1c_Transformants.jpeg" alt="colonies_total" width="500">
-<figcaption>Fig.1 - Transformation of the L1c plasmid in <i>E. coli</i>. The red <i>E. coli</i> colonies obtained the plasmid containing RFP as an selection marker. </figcaption>
+<figcaption>Fig.1 - This part was used for our level 1 c backbone. This image shows a successful transformation of the level 1 c backbone with RFP in <i>E. coli</i>, which makes the colonies red. </figcaption>
 </figure>