Difference between revisions of "Part:BBa K3228054"
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===Parts of the Marburg Toolbox===
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[[File:T--Marburg--5'Con.png|90px|thumb|none|
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<li> <a href="https://parts.igem.org/Part:BBa_K3228054">K3228054 </a> (riboJ B0034) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228055">K3228055 </a> (riboJ B0034 <br> no Scar) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228056">K3228056 </a> <br> (SarJ B0034) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228057">K3228057 </a> (PlmJ B0034) </li>
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Revision as of 16:23, 18 October 2019


pMC_0_3_12_riboJ_B0034

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part contains the ribosomal binding site B0034 and riboJ which is a ribozyme.

Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS). This leads to a more stable expression independent of the cellular context (See iGEM William and Mary 2016) and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.

Parts of the Marburg Toolbox