Difference between revisions of "Part:BBa K3117047"

Latest revision as of 02:08, 22 October 2019

scFv against GPA33 with SpyCatcher codon optimized for E.coli

Part BBa_K3117047 is a fusion protein of an anti-GPA33 single-chain variable fragment (scFv) and a SpyCatcher.

Usage and Biology

By connecting the variable regions of the heavy and light chain of an anti-GPA33 antibody with a short, flexible GGGGS linker (BBa_K3117028), the scFv retains it's antigen-binding ability and is much smaller than a conventional antibody. The SpyCatcher attached to the scFv belongs to the SpyTag/SpyCatcher system, of which the sequence is derived of the FbaB protein in the bacterium Streptococcus pyogenes. Once it comes into contact with its corresponding other part, the SpyTag (BBa_K3117015), they bind covalently (Hatlem et al., 2019).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 54
Illegal AgeI site found at 519
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3117047 short</partinfo>
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+Part BBa_K3117047 is a fusion protein of an anti-GPA33 single-chain variable fragment (scFv) and a SpyCatcher.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+By connecting the variable regions of the heavy and light chain of an anti-GPA33 antibody with a short, flexible GGGGS linker (BBa_K3117028), the scFv retains it's antigen-binding ability and is much smaller than a conventional antibody. The SpyCatcher attached to the scFv belongs to the SpyTag/SpyCatcher system, of which the sequence is derived of the FbaB protein in the bacterium Streptococcus pyogenes. Once it comes into contact with its corresponding other part, the SpyTag (BBa_K3117015), they bind covalently (Hatlem et al., 2019).
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