Difference between revisions of "Part:BBa K3228060"
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This two step assembly heavily cuts down the invested workload and the cost per sample. iGEM Marburg 2019 investigated these advantages on the example of a 20-piece large scale assembly LLLlink to results of placeholdersLLL.
 
This two step assembly heavily cuts down the invested workload and the cost per sample. iGEM Marburg 2019 investigated these advantages on the example of a 20-piece large scale assembly LLLlink to results of placeholdersLLL.
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===Usage===
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Place holders are used in assemblies like any other part in the Marburg Collection 2.0, just make sure to leave out any final digestion step in your Golden Gate reaction cycle to make sure the dropout does not cut itself out. For more information head over to LLLLink to Design Toolbox 2019LLL.
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===Compability===
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs and BsaI as restriction enzyme for “LVL1” like assemblies of single transcription units. For more information we recommend to head over to LLLLink to Design Marburg collection 2018LLL and LLLLink to Design of Toolbox 2019LLL.
  
 
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Revision as of 13:46, 18 October 2019


pMC_0_2_XX_PRO_placeholder

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part is one of the place holders published by iGEM Marburg 2019. These place holder parts can be used in assemblies to subsequently replace it with another part of the same type in a secondary Golden Gate assembly. These parts are very useful if a lot of constructs that only differ in one position are to be constructed. The advantages of using placeholder in high throughput assemblies are clear: A seven part assembly usually requires to screen multiple colonies before finding the right one, meaning that a lot of test digesting or sequencing is involved. This is feasible if one wants to construct only a few parts- for high throughput assemblies the cost and time does not scale well enough. A two part assembly however has an extremely high success rate, meaning that in most cases it is sufficient to just pick one colony to get correct sequencing results.

graphic showing the workflow for a placeholder based assembly

This two step assembly heavily cuts down the invested workload and the cost per sample. iGEM Marburg 2019 investigated these advantages on the example of a 20-piece large scale assembly LLLlink to results of placeholdersLLL.

Usage

Place holders are used in assemblies like any other part in the Marburg Collection 2.0, just make sure to leave out any final digestion step in your Golden Gate reaction cycle to make sure the dropout does not cut itself out. For more information head over to LLLLink to Design Toolbox 2019LLL.

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs and BsaI as restriction enzyme for “LVL1” like assemblies of single transcription units. For more information we recommend to head over to LLLLink to Design Marburg collection 2018LLL and LLLLink to Design of Toolbox 2019LLL.