Difference between revisions of "Part:BBa K143013"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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=== Promoters and Their Primers === | === Promoters and Their Primers === | ||
| − | Promoter P43 is a strong promoter of ''Bacillus subtilis'' expression system. It is derived from cytidine deaminase (cdd) gene in ''B. subtilis ''. It is often used to express different target proteins. | + | '''Promoter P43 is a strong promoter of ''Bacillus subtilis'' expression system. It is derived from cytidine deaminase (cdd) gene in ''B. subtilis ''. It is often used to express different target proteins. |
Several promoters with high expression level that are commonly used in ''Bacillus subtilis'' were screened to determine the most efficient ones for our experiments on the construction of biological parts. Three of such promoters, Groe ([https://parts.igem.org/Part:BBa_J100034 BBa J100034]), SecA ([https://parts.igem.org/Part:BBa_K1469002 BBa K1469002]), P43 (BBa_K143013), exist in the iGEM registry already. | Several promoters with high expression level that are commonly used in ''Bacillus subtilis'' were screened to determine the most efficient ones for our experiments on the construction of biological parts. Three of such promoters, Groe ([https://parts.igem.org/Part:BBa_J100034 BBa J100034]), SecA ([https://parts.igem.org/Part:BBa_K1469002 BBa K1469002]), P43 (BBa_K143013), exist in the iGEM registry already. | ||
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<p>We have thus characterized the basic biobricks Groe (BBa J100034), SecA (BBa K1469002) and P43 (BBa K208002)</p> | <p>We have thus characterized the basic biobricks Groe (BBa J100034), SecA (BBa K1469002) and P43 (BBa K208002)</p> | ||
| − | + | SDS-PAGE electrophoresis results: (band for the ASN protein at 43kDa is highlighted in red color) | |
| − | Column M is the marker, column 1, 2, 3, 4, 5 and 6 are for the Bacillus subtilis constructs with the plasmids of WB0911H-ASN(original plasmid)、WBGroE、WBYxiE、WBSecA、WBYlbP and WB43, respectively. | + | Column M is the marker, column 1, 2, 3, 4, 5 and 6 are for the Bacillus subtilis constructs with the plasmids of WB0911H-ASN(original plasmid)、WBGroE、WBYxiE、WBSecA、WBYlbP and WB43, respectively.''' |
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Revision as of 13:29, 18 October 2019
Promoter 43: Constitutive Promoter for B. subtilis
Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in B.subtilis. This promoter has been shown to be recognized and active during the exponential and lag phases of growth. It has been hypothesized that the ability to recognize the promoter in exponential and lag phase of growth is due to the recognition of the promoter by both sigma factor 55 (the major sigma factor A) and sigma factor 37 (the lag phase sigma factor B) 1. The P43 promoter has been previously used for constitutive expression of exogenous genes within B.subtilis vectors 2. The context with which we used the promoter P43 is as a Polymerase Per Second (PoPS) generator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]