Difference between revisions of "Part:BBa K3040001"
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Lipase A1 from Antarctic deep sea bacterium, Psychrobacter sp 7195., can catalyze the hydrolysis of triacylgly-cerols to glycerol and monoacyl-glycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A1 reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drop. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A. | Lipase A1 from Antarctic deep sea bacterium, Psychrobacter sp 7195., can catalyze the hydrolysis of triacylgly-cerols to glycerol and monoacyl-glycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A1 reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drop. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:35, 19 October 2019
Lipase A1 (Psychrobacter sp 7195.) -Histag
Lipase A1 from Antarctic deep sea bacterium, Psychrobacter sp 7195., can catalyze the hydrolysis of triacylgly-cerols to glycerol and monoacyl-glycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A1 reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drop. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A.
under
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1186