Difference between revisions of "Part:BBa K2915225"
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'''Design Notes'''
 
'''Design Notes'''
 +
 
This biobrick is in RFC 10 standards with:
 
This biobrick is in RFC 10 standards with:
 
the prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC 
 
the prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC 
 
and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
 
and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
 +
 
This TAL1 exists with:
 
This TAL1 exists with:
 +
 
- with a His-tag and a TEV site
 
- with a His-tag and a TEV site
 +
 
- with a promotor T7 and RBS (BBa_K525998) which is the regulator.
 
- with a promotor T7 and RBS (BBa_K525998) which is the regulator.

Revision as of 12:22, 18 October 2019


IPTG inducible promoter (T7) with RBS TAL1 6-His-Tag with TEV site

The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is in RFC 10 standards with: the prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This TAL1 exists with:

- with a His-tag and a TEV site

- with a promotor T7 and RBS (BBa_K525998) which is the regulator.