Difference between revisions of "Part:BBa K3102043"

Revision as of 14:07, 19 October 2019

Lactate production (ldhA, lldP, ACS operon)

This "lactate production" composite part is composed of Lactate dehydrogenase (LdhA):BBa_K3102026 and lactate permease (LldP):BBa_K3102027 sequences. This part increases the production and secretion of D-Lactate in the extracellular media. The LDHA enzyme catalyse the interconversion of pyruvate to L-lactate with simultaneous conversion of NADH to NAD+.

This composite part also constains the ACS operon:BBa_K3102028, composed of three genes: actP (Transports acetate), acs (catalyzes the conversion of acetate into acetyl-CoA), and yfcH (epimerase). It allows for the uptake of acetate molecules in order for the bacteria to do correctly its metabolism and survive.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1478
Illegal XhoI site found at 3404
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 489
Illegal AgeI site found at 1614
Illegal AgeI site found at 2077
Illegal AgeI site found at 2515
Illegal AgeI site found at 2674
Illegal AgeI site found at 2686
Illegal AgeI site found at 3833
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 219
Illegal SapI.rc site found at 6698

@@ Line 10: / Line 10: @@
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+Comparison of lactate secretion between engineered and non-engineered bacteria.
+An important step in our system in the optimization of lactate secretion by E. coli. Indeed, lactate constitute the energetic substrate of S. oneidensis and thus an essential element to the generation of electricity.
+Lactate detection kit was used as described in “Protocol: Lactate Detection” and the following measurement were recovered:
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