Difference between revisions of "Part:BBa K2908681:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The initiation codon ATG can only appear between miRNA sponse and EGFP.
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The initiation codon ATG can only appear between miRNA sponse and EGFP.<br/>
 
- The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture.
 
- The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture.
 
https://static.igem.org/mediawiki/parts/7/78/T--CSU_CHINA--ggmodule2.png
 
https://static.igem.org/mediawiki/parts/7/78/T--CSU_CHINA--ggmodule2.png
−
 
−
 
  
 
===Source===
 
===Source===

Revision as of 21:47, 17 October 2019


s(ESR1)p-miR101spe-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 200
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initiation codon ATG can only appear between miRNA sponse and EGFP.
- The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture. T--CSU_CHINA--ggmodule2.png

Source

We designed it by ourselves and constructed this part with the help of Golden gate.

References