Difference between revisions of "Part:BBa K2908671"

Revision as of 14:21, 21 October 2019

pLN431-s(GATA3)p-GAD-miR101-BS

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of GAD and miR101-BS for sequence-specific expression of GAD.Therefore,we used our p(GATA3)p(BBa_K2908000) as a specific promoter. At the same time we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation domain of GAD.In this way we can ensure the specificity and independence of this protein. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 99
Illegal NheI site found at 584
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 360
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 279

@@ Line 4: / Line 4: @@
 The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of GAD and miR101-BS for sequence-specific expression of GAD.Therefore,we used our p(GATA3)p(BBa_K2908000) as a specific promoter. At the same time we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101  and fuse it to 3' end so that can regulate the transactivation domain of GAD.In this way we can ensure the specificity and independence of this protein.
-https://static.igem.org/mediawiki/parts/8/82/T--CSU_CHINA--module1.png
+https://2019.igem.org/wiki/images/5/50/T--CSU_CHINA--2908671.jpg
 <!-- Add more about the biology of this part here
 ===Usage and Biology===