Difference between revisions of "Part:BBa K145201:Experience"
(→User Reviews) |
(→User Reviews) |
||
| Line 16: | Line 16: | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
The 2008 KUleuven team has tested this part. We added a GFP under TetR promoter to this part. Since TetR was constitutively produced, fluorescence was detected. This part works very good. More information can be found [http://2008.igem.org/Team:KULeuven/Data/Input here]. | The 2008 KUleuven team has tested this part. We added a GFP under TetR promoter to this part. Since TetR was constitutively produced, fluorescence was detected. This part works very good. More information can be found [http://2008.igem.org/Team:KULeuven/Data/Input here]. | ||
| + | |}; | ||
| + | |||
| + | <!-- DON'T DELETE --><partinfo>BBa_K145201 EndReviews</partinfo> | ||
| + | |||
| + | |||
| + | ===User Reviews=== | ||
| + | <!-- DON'T DELETE --><partinfo>BBa_K145201 StartReviews</partinfo> | ||
| + | <!-- Template for a user review | ||
| + | <!-- End of the user review template --> | ||
| + | {|width='80%' style='border:1px solid gray' | ||
| + | |- | ||
| + | |width='10%'| | ||
| + | <partinfo>BBa_K145201 AddReview 3</partinfo> | ||
| + | <I>BNathalie</I> | ||
| + | |width='60%' valign='top'| | ||
| + | The iGEM Bulgaria 2017 team used this part. We added a GFP or RFP under Ptet promoter to this part. TetR is constitutively produced, but still some basal fluorescence was detected in an uninduced samples (left tube on the picture below, the right one is induced). | ||
| + | |||
| + | https://i.imgur.com/3shbtjA.jpg | ||
| + | |||
| + | After careful sequence analysis we discovered a LVA-like tag fused to TetR that is not mentioned into the part's description. | ||
| + | |||
| + | We generated a new part that is without the degradation tag and is in inverted orientation. You can have a look here: https://parts.igem.org/Part:BBa_K2515003 | ||
|}; | |}; | ||
<!-- DON'T DELETE --><partinfo>BBa_K145201 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K145201 EndReviews</partinfo> | ||
Latest revision as of 21:54, 1 November 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K145201
User Reviews
UNIQadf6422d93ff8147-partinfo-00000000-QINU
|
•••••
BNathalie |
The 2008 KUleuven team has tested this part. We added a GFP under TetR promoter to this part. Since TetR was constitutively produced, fluorescence was detected. This part works very good. More information can be found [http://2008.igem.org/Team:KULeuven/Data/Input here]. |
UNIQadf6422d93ff8147-partinfo-00000002-QINU
User Reviews
UNIQadf6422d93ff8147-partinfo-00000003-QINU
|
•••
BNathalie |
The iGEM Bulgaria 2017 team used this part. We added a GFP or RFP under Ptet promoter to this part. TetR is constitutively produced, but still some basal fluorescence was detected in an uninduced samples (left tube on the picture below, the right one is induced).
After careful sequence analysis we discovered a LVA-like tag fused to TetR that is not mentioned into the part's description. We generated a new part that is without the degradation tag and is in inverted orientation. You can have a look here: https://parts.igem.org/Part:BBa_K2515003 |
UNIQadf6422d93ff8147-partinfo-00000005-QINU