Difference between revisions of "Part:BBa K2908679"
| Line 4: | Line 4: | ||
The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR141 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR141-sponge(BBa_K2908555) to bind different concentrations of miR141 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein. | The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR141 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR141-sponge(BBa_K2908555) to bind different concentrations of miR141 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein. | ||
| − | + | https://static.igem.org/mediawiki/parts/a/a2/T--CSU_CHINA--module2141spe.png | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 21:43, 17 October 2019
s(ESR1)p-miR141spe-EGFP
The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR141 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR141-sponge(BBa_K2908555) to bind different concentrations of miR141 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 155
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 200
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]