Difference between revisions of "Part:BBa K245129"

(→‎Usage and Biology)
(→‎Usage and Biology)
Line 20: Line 20:
 
              
 
              
 
<h3>Modeling the protein binding to sodium ion with c-score: 0.1</h3>
 
<h3>Modeling the protein binding to sodium ion with c-score: 0.1</h3>
−
                https://2019.igem.org/wiki/images/e/e1/T--CU--2.gif
+
https://2019.igem.org/wiki/images/e/e1/T--CU--2.gif
  
  

Revision as of 16:27, 17 October 2019

CutA1

This part is the coding sequence for a trimerization domain - CutA1.

Usage and Biology

In silico protein modeling

Using Expasy ProtParam tool*, theoretical protein extinction coefficient has been identified in addition to several other factors that predict the protein stability, pI and halophilicity.

T--CU--Table1.png

After which we aimed to identify the protein 3D structure and which ligand it binds by using molecular dynamics protein function prediction tool which is I-tasser. Id of the work submitted is: S494196

Predicted Model with the least free energy

T--CU--1.gif


The biological process identified in the model in response to metal ion, through the ligand binding site prediction, we were able to predict that CutA could bind to sodium ion;Gene ontology predicted in the model belonged to copper binding.

Modeling the protein binding to sodium ion with c-score: 0.1

T--CU--2.gif


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]