Difference between revisions of "Part:BBa K245129"
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After which we aimed to identify the protein 3D structure and which ligand it binds by using molecular dynamics protein function prediction tool which is I-tasser. | After which we aimed to identify the protein 3D structure and which ligand it binds by using molecular dynamics protein function prediction tool which is I-tasser. | ||
Id of the work submitted is: S494196 | Id of the work submitted is: S494196 | ||
| − | + | <h2>Predicted Model with the least free energy</h2> | |
https://2019.igem.org/wiki/images/0/05/T--CU--1.gif | https://2019.igem.org/wiki/images/0/05/T--CU--1.gif | ||
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The biological process identified in the model in response to metal ion, through the ligand binding site prediction, we were able to predict that CutA could bind to sodium ion;Gene ontology predicted in the model belonged to copper binding. | The biological process identified in the model in response to metal ion, through the ligand binding site prediction, we were able to predict that CutA could bind to sodium ion;Gene ontology predicted in the model belonged to copper binding. | ||
| + | <h3>Modeling the protein binding to sodium ion with c-score: 0.1</h3> | ||
| + | https://2019.igem.org/wiki/images/e/e1/T--CU--2.gif | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 16:25, 17 October 2019
CutA1
This part is the coding sequence for a trimerization domain - CutA1.
Usage and Biology
In silico protein modeling
Using Expasy ProtParam tool*, theoretical protein extinction coefficient has been identified in addition to several other factors that predict the protein stability, pI and halophilicity.
After which we aimed to identify the protein 3D structure and which ligand it binds by using molecular dynamics protein function prediction tool which is I-tasser. Id of the work submitted is: S494196
Predicted Model with the least free energy
The biological process identified in the model in response to metal ion, through the ligand binding site prediction, we were able to predict that CutA could bind to sodium ion;Gene ontology predicted in the model belonged to copper binding.
Modeling the protein binding to sodium ion with c-score: 0.1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]