Difference between revisions of "Part:BBa K145001"
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This RNA polymerase will start transcription from a T7 promoter (such as [https://parts.igem.org/Part:BBa_I712074 BBa_I712074]). | This RNA polymerase will start transcription from a T7 promoter (such as [https://parts.igem.org/Part:BBa_I712074 BBa_I712074]). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <h2> <b> BOKU-Vienna 2019 - Improvement </b> </h2> | ||
+ | |||
+ | Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, the Team BOKU-Vienna decided to remove those recognition sites by codon swapping to improve the applicability, you can find the functioning Polymerase under [[Part:BBa_K3015006|BBa_K3015006]] | ||
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Revision as of 12:26, 19 October 2019
T7 RNA polymerase
This RNA polymerase will start transcription from a T7 promoter (such as BBa_I712074).
Usage and Biology
BOKU-Vienna 2019 - Improvement
Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, the Team BOKU-Vienna decided to remove those recognition sites by codon swapping to improve the applicability, you can find the functioning Polymerase under BBa_K3015006
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]