Difference between revisions of "Part:BBa K245129"
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===Usage and Biology===
 
===Usage and Biology===
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<h1><b>In silico protein modeling</b></h1>
 
<h1><b>In silico protein modeling</b></h1>
 
<p>Using Expasy ProtParam tool*, theoretical protein extinction coefficient has been identified in addition to several other factors that predict the protein stability, pI and halophilicity.</p>
 
<p>Using Expasy ProtParam tool*, theoretical protein extinction coefficient has been identified in addition to several other factors that predict the protein stability, pI and halophilicity.</p>
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<table>
 
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<tr>
 
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<th scope="col">Extinction coefficient</th>
 
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<th scope="col"> Gravy</th>
 
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</tr>
 
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<th scope="row">20690</th>
 
  
  
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Id of the work submitted is: S494196  
 
Id of the work submitted is: S494196  
  
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https://2019.igem.org/wiki/images/0/05/T--CU--1.gif
 +
                 
 +
 +
                      predicted Model with the least free energy
 +
 +
 +
The biological process identified in the model in response to metal ion, through the ligand binding site prediction, we were able to predict that CutA could bind to sodium ion;Gene ontology predicted in the model belonged to copper binding.
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                https://2019.igem.org/wiki/images/e/e1/T--CU--2.gif
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 15:35, 17 October 2019

CutA1

This part is the coding sequence for a trimerization domain - CutA1.

Usage and Biology

In silico protein modeling

Using Expasy ProtParam tool*, theoretical protein extinction coefficient has been identified in addition to several other factors that predict the protein stability, pI and halophilicity.


After which we aimed to identify the protein 3D structure and which ligand it binds by using molecular dynamics protein function prediction tool which is I-tasser. Id of the work submitted is: S494196

T--CU--1.gif 


                     predicted Model with the least free energy


The biological process identified in the model in response to metal ion, through the ligand binding site prediction, we were able to predict that CutA could bind to sodium ion;Gene ontology predicted in the model belonged to copper binding. 


               T--CU--2.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]