Difference between revisions of "Part:BBa K3226019"
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| − | This coding sequence has 6×histidine attached to RecA was designed by iGEM11_Osaka. This coding sequence is needed to make device (BBa_K3226020). The following sentences are mainly related to RecA. | + | This coding sequence has 6×histidine (BBa_K2888600) attached to RecA was designed by iGEM11_Osaka. This coding sequence is needed to make device (BBa_K3226020). The following sentences are mainly related to RecA. |
Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions. | Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions. | ||
Latest revision as of 05:04, 18 October 2019
RecA+his
<Long description>
This coding sequence has 6×histidine (BBa_K2888600) attached to RecA was designed by iGEM11_Osaka. This coding sequence is needed to make device (BBa_K3226020). The following sentences are mainly related to RecA.
Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions.
<Usage>
This is mainly used to obtain resistance to radiation. In our project, we used γ-rays as one type of radiation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 242
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 916
- 1000COMPATIBLE WITH RFC[1000]