Difference between revisions of "Part:BBa K3226020:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We made this parts by using in-fusion cloning. The homologous sequence is needed between insert with vector to do in-fusion cloning. In this way, we took care not to make a mistake in the operation part of the infusion cloning that there is not in the restriction enzyme operation. Please refer to the dedicated page for detailed protocol. | |
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===Source=== | ===Source=== | ||
Revision as of 15:04, 17 October 2019
Add the his tag to BBa_K602008.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 3396
Illegal suffix found in sequence at 1348 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3396
Illegal SpeI site found at 1349
Illegal PstI site found at 1363
Illegal NotI site found at 1356
Illegal NotI site found at 3402 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3396
Illegal BglII site found at 468
Illegal XhoI site found at 2380
Illegal XhoI site found at 3272 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 3396
Illegal suffix found in sequence at 1349 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 3396
Illegal XbaI site found at 3411
Illegal SpeI site found at 1349
Illegal PstI site found at 1363
Illegal NgoMIV site found at 1142 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We made this parts by using in-fusion cloning. The homologous sequence is needed between insert with vector to do in-fusion cloning. In this way, we took care not to make a mistake in the operation part of the infusion cloning that there is not in the restriction enzyme operation. Please refer to the dedicated page for detailed protocol.
Source
Deinococcus Radiodurans
References
Tomoyasu Aizawa., (2008)., Ni-NTA affinity column., Protein Science Society archive#019., Protein Science Society of Japan.