Difference between revisions of "Part:BBa K2984022"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2984022 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2984022 SequenceAndFeatures</partinfo> | ||
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+ | ===Usage and Biology=== | ||
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+ | For the effective and regulated expression of genes a promoter is required. The promoter is located at the 5’ region of the sense strand of the DNA and provides a binding site for the RNA-polymerase and transcription factors. In the nucleus of the green alga Chlamydomonas reinhardtii a gene encoding a chloroplast protein - PsaD - is located. Fischer and Rochaix (2001) showed that the ORF of this gene does not contain any introns and that the promoter drives a strong constitutive expression of the gene. Using the identified sequence of the promoter a vector for the high-level expression of endogenous and exogenous genes was created (Fischer and Rochaix, 2001). | ||
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+ | ==References== | ||
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+ | Fischer, N., & Rochaix, J. D. (2001). The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii. Molecular Genetics and Genomics, 265(5), 888-894. | ||
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Revision as of 18:20, 18 October 2019
PsaD Promoter A1-A3; High-level expression of genes
This part is a part of the Chlamy-HUB-Collection. For the effective and regulated expression of genes a promoter is required. The promoter is located at the 5’ region of the sense strand of the DNA and provides a binding site for the RNA-polymerase and transcription factors. In the nucleus of the green alga Chlamydomonas reinhardtii a gene encoding a chloroplast protein - PsaD - is located. Fischer and Rochaix (2001) showed that the ORF of this gene does not contain any introns and that the promoter drives a strong constitutive expression of the gene. Using the identified sequence of the promoter a vector for the high-level expression of endogenous and exogenous genes was created (Fischer and Rochaix, 2001).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 18
Illegal XhoI site found at 842 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
For the effective and regulated expression of genes a promoter is required. The promoter is located at the 5’ region of the sense strand of the DNA and provides a binding site for the RNA-polymerase and transcription factors. In the nucleus of the green alga Chlamydomonas reinhardtii a gene encoding a chloroplast protein - PsaD - is located. Fischer and Rochaix (2001) showed that the ORF of this gene does not contain any introns and that the promoter drives a strong constitutive expression of the gene. Using the identified sequence of the promoter a vector for the high-level expression of endogenous and exogenous genes was created (Fischer and Rochaix, 2001).
References
- Fischer, N., & Rochaix, J. D. (2001). The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii. Molecular Genetics and Genomics, 265(5), 888-894.