Difference between revisions of "Part:BBa K2984017"
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<partinfo>BBa_K2984017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984017 SequenceAndFeatures</partinfo>
  
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==Characterization==
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This part was characterized as a part of construct BBa_K2984019. For more information and the characterization data please click <a href="https://parts.igem.org/Part:BBa_K2984019">here</a>.
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Revision as of 18:02, 18 October 2019


YFP - Yellow fluorescent protein for detection B3-B4

This part is a part of the Chlamy-HUB-Collection. For in vivo detection of proteins in C. reinhardtii or its surroundings. The green fluorescent protein variant carries amino acid substitutions that shift the protein to which it is bound to an emission peak at 530 nm seen as yellow (Ormo et al., 1996). For detection of fluorescence it is not necessary to process a sample nor adding a substance to make it visible making it safe for C. reinhardtii as it can be detected with a fluorescence microscope.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

This part was characterized as a part of construct BBa_K2984019. For more information and the characterization data please click here.