Difference between revisions of "Part:BBa K2984000"
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. For the secretion of a protein in <i>Chlamydomonas reinhardtii</i> it is necessary that this protein is attached to a signal sequence that targets it to the secretory pathway and eventually into the medium. It has been demonstrated that the Arylsulfatase 1 (ARS1) is not only able to direct protein synthesis to the secretory pathway, it was also shown that it is able to secrete heterologous proteins (Rasala et al., 2012). The amino acid sequence of the signal peptide of ARS1 is MHARKMGALAVLAVACLAAVASVAHA.
 
This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. For the secretion of a protein in <i>Chlamydomonas reinhardtii</i> it is necessary that this protein is attached to a signal sequence that targets it to the secretory pathway and eventually into the medium. It has been demonstrated that the Arylsulfatase 1 (ARS1) is not only able to direct protein synthesis to the secretory pathway, it was also shown that it is able to secrete heterologous proteins (Rasala et al., 2012). The amino acid sequence of the signal peptide of ARS1 is MHARKMGALAVLAVACLAAVASVAHA.
 
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<partinfo>BBa_K2984000 SequenceAndFeatures</partinfo>
 
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<img src="https://2019.igem.org/wiki/images/f/f0/T--Humboldt_Berlin--WhenARSworks.jpeg" alt="colonies_total" width="500">
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<figcaption>Fig.1 - <i>C. reinahrdtii </i> colonies on TAP-Agar plate. The blue colonies indicate a successfull incision inside the SNRK locus which regulates the expression of the arylsulfatase. The blue color is generated through the activity of the arylsulfatase outside the cells, verifiying the functionality of the ARS secretion signal.</figcaption>
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Revision as of 16:23, 18 October 2019


3'UTR, Secretion signal of Arylsulfatase ARS B2-B2

This vector is a part of the Chlamy-HUB-Collection. For the secretion of a protein in Chlamydomonas reinhardtii it is necessary that this protein is attached to a signal sequence that targets it to the secretory pathway and eventually into the medium. It has been demonstrated that the Arylsulfatase 1 (ARS1) is not only able to direct protein synthesis to the secretory pathway, it was also shown that it is able to secrete heterologous proteins (Rasala et al., 2012). The amino acid sequence of the signal peptide of ARS1 is MHARKMGALAVLAVACLAAVASVAHA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

colonies_total
Fig.1 - C. reinahrdtii colonies on TAP-Agar plate. The blue colonies indicate a successfull incision inside the SNRK locus which regulates the expression of the arylsulfatase. The blue color is generated through the activity of the arylsulfatase outside the cells, verifiying the functionality of the ARS secretion signal.